Journal: Biomolecules

Article Title: Integrated Proteomic and Metabolomic Analysis of Muscle Atrophy Induced by Hindlimb Unloading.

doi: 10.3390/biom15010014

Figure Lengend Snippet: Figure 3. Effect of hindlimb unlaoding on muscle fiber type. (A) Violin plots showing DEPs related to myofiber type. (B) The immunofluorescence assays of fast-twitch fibers (green) and slow-twitch muscle fiber types (red). (C,D) Western blot and Immunofluorescent staining with target ACTN3 and YAP1. (Scale Bar = 50 µm. The data represent the mean ± SEM, n = 4; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).Western blot original images can be found in Figure S5.

Article Snippet: The membranes were then incubated with antibodies against ACTN3 (Proteintech, 1:2000, Wuhan, China), GLUL (Proteintech, 1:4000, Wuhan, China), GSTM4 (Proteintech, 1:2000, Wuhan, China), FBP2 (Proteintech, 1:2000, Wuhan, China), YAP1 (Proteintech, 1:5000, Wuhan, China), NDUFS4 (Proteintech, 1:2000, Wuhan, China), and GAPDH (Zhuangzhi, 1:2000, Xian, China) allowing for overnight incubation at 4 ◦C.

Techniques: Immunofluorescence, Western Blot, Staining

Journal: Biomolecules

Article Title: Integrated Proteomic and Metabolomic Analysis of Muscle Atrophy Induced by Hindlimb Unloading.

doi: 10.3390/biom15010014

Figure Lengend Snippet: Figure 6. Integrated analyses of Proteomics and Metabolomics. (n = 3) (A) Venn diagrams showing the number of protein (blue) and metabolite (red) pathways significantly changed after hindlimb unloading, followed by a list of pathways that were regulated at both the protein and metabolite levels. (B) Barplot of KEGG pathway enrichment. (C,D) The KEGG pathway maps of lysosomes (C) and ferroptosis (D). (Blue indicates downregulation and red indicates upregulation. The small disc represents metabolites. The rectangle represents proteins.) (E) Heat map of correlation between DEPs ACTN3, FBP2, GLUL, GSTM4, NDUFS4, YAP1, and metabolites. (* p < 0.05, ** p < 0.01).

Article Snippet: The membranes were then incubated with antibodies against ACTN3 (Proteintech, 1:2000, Wuhan, China), GLUL (Proteintech, 1:4000, Wuhan, China), GSTM4 (Proteintech, 1:2000, Wuhan, China), FBP2 (Proteintech, 1:2000, Wuhan, China), YAP1 (Proteintech, 1:5000, Wuhan, China), NDUFS4 (Proteintech, 1:2000, Wuhan, China), and GAPDH (Zhuangzhi, 1:2000, Xian, China) allowing for overnight incubation at 4 ◦C.

Techniques:

Journal: The FASEB Journal

Article Title: Overexpression of enhanced yellow fluorescent protein fused with Channelrhodopsin‐2 causes contractile dysfunction in skeletal muscle

doi: 10.1096/fj.202401664RR

Figure Lengend Snippet: Western blot antibody information.

Article Snippet: ACTN3 (ProteinTech, #24378‐1‐AP) 1:1000 , Goat anti‐rabbit (ProteinTech #SA00001‐2) 1:6000.

Techniques: Western Blot

Journal: The FASEB Journal

Article Title: Overexpression of enhanced yellow fluorescent protein fused with Channelrhodopsin‐2 causes contractile dysfunction in skeletal muscle

doi: 10.1096/fj.202401664RR

Figure Lengend Snippet: Western blot antibody information.

Article Snippet: ACTN3 (ProteinTech, #24378‐1‐AP) 1:1000 , Goat anti‐rabbit (ProteinTech #SA00001‐2) 1:6000.

Techniques: Western Blot

Journal: The FASEB Journal

Article Title: Overexpression of enhanced yellow fluorescent protein fused with Channelrhodopsin‐2 causes contractile dysfunction in skeletal muscle

doi: 10.1096/fj.202401664RR

Figure Lengend Snippet: Presence of EYFP led to reduced HCN2 and FBP2 protein levels in gastrocnemius skeletal muscle. Significantly reduced protein‐level expressions of (A) HCN2 in the ChR2‐EYFP muscle and (B) FBP2 in both ChR2‐EYFP and ChR2‐only muscles were observed, compared with ChR2‐only and WT muscles, respectively. (C) ACTN3 protein expression was significantly higher in ChR2‐only muscle compared with WT and ChR2‐EYFP muscles. Error bars denote means ± SD. WT group included n = 1 ChR2‐EYFP fl/fl and n = 2 ChR2‐only fl/fl mice (all cre‐negative).

Article Snippet: ACTN3 (ProteinTech, #24378‐1‐AP) 1:1000 , Goat anti‐rabbit (ProteinTech #SA00001‐2) 1:6000.

Techniques: Muscles, Expressing